For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Modified version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI^®Pol TaqHY is recommended for use in all PCR applications where very short cycle periods or particularly high yields are required. This polymerase set ROTI^®Pol TaqHY is perfectly suitable for all Taq-based cycling protocols with a) high yield, as being performed, for instance, prior to cloning, for amplicon elution, when using low-copy templates, or for educational purposes, or if b) particularly short cycle times are required. ROTI^®Pol TaqHY has been engineered for enhanced polymerase activity as well as for particularly speedy elongation, resulting in enhanced yields as well as in time-saving short elongation times. In combination with our unique buffers, the TaqHY polymerase delivers specific PCR amplification with a wide range of PCR templates. Using shorter cycle times, the amplicon amount resulting from this reaction is significantly higher as known from standard Taq polymerases. ROTI^®Pol TaqHY is able to amplify PCR products up to 3 kb with genomic DNA and is appropriate for use in the amplification of DNA from a wide range of even complex templates. The TaqHY DNA polymerase included in the set possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

Filled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl₂, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.

TaqHY polymerase in storage buffer containing 50 % glycerol, PCR buffer (10x) with 20 mM MgCl₂, PCR buffer red (10x) with 20 mM MgCl₂ and 0,1 % cresol red. Colour coded tubes.Contents of this set may not be bought separately.