Recombinant Human GDNF Protein (Active)

Sizes: 20μg, 100μg

Catalogue Numbers: PKSH032488-20, PKSH032488-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: GDNF

Target Symptom: Glial Cell Line-Derived Neurotrophic Factor; hGDNF; Astrocyte-Derived Trophic Factor; ATF; GDNF

Research Area: Neuroscience; Developmental Biology

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion Tag: C-His

UNIProt ID: P39905

Accession: P39905

Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.

Activity: Measure by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is 10 ng/mL.

Sequence: Ser78-Ile211

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 15.1 kDa

Observed MW: 17 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only