Symmetric DiMethyl-Histone H3-R26 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8453-50, BS8453-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P68431

Host: Rabbit

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Methylated Antibodies

Bioworld Molecular Weight: 17kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R26 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R26 antibody., Immunofluorescence analysis of U-2 OS cells using Symmetric DiMethyl-Histone H3-R26 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: HIST1H3J; H3/j; H3FJ

Immunogen: A synthetic symmetric dimethylated peptide around R26 of human histone H3(NP_003520.1).

Conjugate: Unconjugated

Modification: Methylated