Flag-Tag (HRP Conjugated) Mouse mAb

Sizes: 50μL, 100μL

Catalogue Numbers: EM1024-50, EM1024-100

Citations, Manuals and MSDS Available upon request.

Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Alternate Name: DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag

Source: Mouse

Applications: WB

Dilution: WB 1:5, 000-10, 000

Immunogen: Synthetic Peptide

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality: Monoclonal

Isotype: IgG1

Concentration: 1mg/mL

Research Use Only