RBFOX3 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-70331-60, E-AB-70331-120, E-AB-70331-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: RBFOX3

Target Synonym: FLJ56884; FLJ58356; Fox-1 homolog C; fox1 homolog C; Fox3; FOX3NeuN; hexaribonucleotide binding protein 3; HRNBP3; NEUN; neuronal nuclei; Rbfox3; RFOX3; RNA binding protein fox-1 homolog 3; RNA binding protein; fox 1 homolog (C. elegans) 3

Research Areas: Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags and Cell Markers

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: A6NFN3,Q8BIF2

Background: Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.

Concentration: 1 mg/mL

Immunogen: Recombinant protein corresponding to Mouse NEUN

Buffer: PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 46-48kDa

Observed MW: 46-48kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only