Histone H1 (P13) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1655-50, BS1655-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q02539

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IP,

All Applications: WB: 1:500~1:1000
IP: 1:50~1:200

Background: Histones are evolutionarily conserved proteins that play a vital role in the compaction, storage, and regulation of DNA within the eukaryotic nucleus. The basic subunit of chromatin, the nucleosome core particle, is composed of DNA wound around two copies each of the core histone proteins H2A, H2B, H3, and H4. Formation of higher order chromatin structure is facilitated through the binding of linker histone H1 to the nucleosome particle (chromatosome). In humans and mice, there are 11 distinct histone H1 variants, which include the somatic variants (H1.1, H1.2, H1.3, H1.4, and H1.5) that are expressed ubiquitously, and cell type specific variants such as H1t found in the testis and H1.0 expressed in terminally differentiated cells. Binding of histone H1 to chromatin limits accessibility of DNA to other proteins by stabilizing nucleosome positioning, competing for binding sites, and limiting the activity of chromatin remodeling proteins such as the SWI/SNF complex. Histone H1 binding is highly dynamic and is thought to be regulated by post-translational modifications. For example, cell cycle regulated phosphorylation of histone H1 leads to chromatin condensation and decondensation depending on the site of phosphorylation and histone H1.4 Lys34 acetylation by GCN5 has been linked to increased mobility of H1.4 and transcriptional activation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Histone H1 (P13) polyclonal antibody detects endogenous levels of Histone H1 protein.

Bioworld Molecular Weight: ~ 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Histone H1 (P13) polyclonal antibody at 1:500 dilution
Lane 1: A2780 whole cell lysate (30ug)
Lane 2: PC12 whole cell lysate (30ug)
Lane 3: HEK293T whole cell lysate (30ug)
Lane 4: Hela whole cell lysate (30ug), Immunoprecipitation of HEK293T cell lysates using Histone H1 (P13) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using Histone H1 (P13) pAb.

Alternative Name: Histone H1.1; Histone H1a; H1-1; H1F1; HIST1H1A

Immunogen: Synthetic peptide, corresponding to Human Histone H1.

Conjugate: Unconjugated

Modification: Unmodified