VPS4B Polyclonal Antibody

Catalogue Numbers: BS61477-50, BS61477-100

Sizes: 50µl, 100µl

Alternative Name: Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75351

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Class E vacuolar protein sorting (vps) proteins are necessary for appropriate sorting of receptors in the yeast endocytic pathway. The yeast Vps4p is a member of the AAA protein family (ATPases associated with diverse cellular activities) and plays an important role in transporting proteins out of a prevacuolar endosomal compartment. In human, two non-allelic orthologous proteins (VPS4A and VPS4B) of yeast Vps4p are known and can form heteromeric complexes with each other. Both VPS4 (also known as SKD1 in mouse) proteins are class E VPSs and are involved in intracellular protein trafficking, similar to Vps4p in yeast. A human CHMP1 protein, which is implicated in multivesicular body formation, physically interacts with VPS4. HIV-1 uses cellular machinery to bud from infected cells and requires VPS4 and TSG101/VPS23 for this budding process. Dominant negative mutant of VPS4 inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Thus, retroviruses normally use the VPS pathway to form multivesicular bodies during the budding process.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: VPS4B polyclonal antibody detects endogenous levels of VPS4B protein.

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of VPS4B polyclonal antibody at 1:1000 dilution
Lane 1: HEK293T whole cell lysate (57μg)
Lane 2: The Lung tissue lysate of Rat (39μg)
Lane 3: Raw264.7 whole cell lysate (51μg)

Immunogen: A synthetic peptide corresponding to residues in Human VPS4B.

Conjugate: Unconjugated

Modification: Unmodified