ARHGDIG Polyclonal Antibody

Catalogue Numbers: BS5899-50, BS5899-100

Sizes: 50µl, 100µl

Alternative Name: Rho GDP-dissociation inhibitor 3; Rho GDI 3; Rho-GDI gamma; Rho GDIγ; Rho-GDIγ

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99819

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTPbound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIα, Ly-GDI (also known as Rho GDIβ and previously known as GDI/D4) and Rho GDIγ. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ARHGDIG polyclonal antibody detects endogenous levels of ARHGDIG protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ARHGDIG polyclonal antibody at 1:500 dilution
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: Raw264.7 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 176-221 of Human ARHGDIG.

Conjugate: Unconjugated

Modification: Unmodified