BML-284 hydrochloride is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM.

Product information

CAS Number: 853220-52-7

Molecular Weight: 350.37

Formula: C19H18N4O3

Chemical Name: N4-(benzo[d][1, 3]dioxol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2, 4-diamine

Smiles: COC1=CC(=CC=C1)C1=CC(NCC2C=C3OCOC3=CC=2)=NC(N)=N1

InChiKey: FABQUVYDAXWUQP-UHFFFAOYSA-N

InChi: InChI=1S/C19H18N4O3/c1-24-14-4-2-3-13(8-14)15-9-18(23-19(20)22-15)21-10-12-5-6-16-17(7-12)26-11-25-16/h2-9H,10-11H2,1H3,(H3,20,21,22,23)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : ≥ 100 mg/mL (285.41 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

BML-284 (10 µM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group. BML-284 (10 µM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen.

In Vivo:

BML-284 hydrochloride (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos.

References:

1. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90.
2. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824.
3. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889

Products are for research use only. Not for human use.