Arrestin-β-1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9935M-50, BS9935M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49407

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF IHCFC

All Applications: WB: 1:1000-1:2000 ICC/IF: 1:50-1:200
IHC: 1:50-1:100 FC: 1:50-1:100

Background: Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Arrestin-β-1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Arrestin-β-1 Rabbit mAb at 1:1000 dilution
Lane 1: PC12 whole cell lysate
Lane 2: Jurkat whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-beta Arrestin 1 antibody.Counter stained with hematoxylin., ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: ARB 1; ARB1; ARR 1; ARR1; ARRB 1; ARRB1; Arrestin 2; Arrestin beta 1; Arrestin beta-1; Beta-arrestin-1; Arrestin-β1; Arrestin β1; β-arrestin-1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified