Recombinant Human Mesothelin/MSLN Protein (aa 296-580, Fc Tag)

Size: 100μg

Catalogue Number: PKSH030720-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Mesothelin, MSLN

Target Synonym: Megakaryocyte potentiating factor, mesothelin, Pre-pro-megakaryocyte-potentiating factor, soluble MPF mesothelin related protein, CAK1, MPF, MSLN, SMR, CAK1, CAK1 antigen

UNIProt ID: Q13421

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: N-hFc

Accession: Q13421-2

Background: Megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised by several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.

Sequence: Glu296-Gly580

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 60.7 kDa

Observed MW: 65 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only