VP16 tagPolyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65868-50, BS65868-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P04486

Host: Rabbit

Reactivity: Human herpesvirus 1

Applications: WB

All Applications: WB=1:500-2000

Background: HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -41°C long term. Avoid freeze-thaw cycles.

Specificity: VP16 tag Polyclonal Antibody detects endogenous levels of VP16 tag protein.

Bioworld Molecular Weight: ~51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-VP16 tag at 1/1000 dilution

Alternative Name: VP16_HHV1F; UL48; Tegument protein VP16; Alpha trans-inducing protein; Alpha-TIF; ICP25; Vmw65

Immunogen: KLH conjugated synthetic peptide derived from HSV-1 VP16:391-490/490aa

Conjugate: Unconjugated

Modification: Unmodified