Mouse LFA1a (Lymphocyte Function Associated Antigen 1 Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4957-48, ELK4957-96, ELK4957-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD11a; ITG-AL; LFA-1; LFA1-A; ITGAL; Integrin,Alpha L; CD11 antigen-like family member A; Leukocyte function-associated molecule 1 alpha chain

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LFA1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LFA1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LFA1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LFA1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only