DTNB (Ellman’s Reag, Ellmans Reagenz, 5,5′-Dithiobis(2-nitrobenzoic acid), 5,5′-Dithiobis-2-nitrobenzoesäure) is a non-fluorescent probe used to quantify the number or concentration of thiol groups in a sample. DTNB is also an allosteric inhibitor of dengue virus protease (NS2B-NS3pro) and Streptomyces proteases.

Product information

CAS Number: 69-78-3

Molecular Weight: 396.35

Formula: C14H8N2O8S2

Synonym:

Ellman’s Reag

Ellmans Reagenz

5, 5′-Dithiobis(2-nitrobenzoic acid)

5, 5′-Dithiobis-2-nitrobenzoesäure

Chemical Name: 5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid

Smiles: [O-][N+](=O)C1=CC=C(C=C1C(O)=O)SSC1=CC(=C(C=C1)[N+]([O-])=O)C(O)=O

InChiKey: KIUMMUBSPKGMOY-UHFFFAOYSA-N

InChi: InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO 79 mg/mL (199.32 mM) Ethanol 79 mg/mL (199.32 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

References:

1. Mouna Ben Elhoul, et al. Int J Biol Macromol. 2015 Aug;79:871-82.
2. Maureen E Hill, et al. Biochemistry. 2019 Feb 12;58(6):776-787.

Products are for research use only. Not for human use.