FGFR2/CD332 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9029-50, MB9029-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21802

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, ICC IHC

All Applications: WB: 1:500
IHC/ICC: 1:100-1:200

Background: Fibroblast growth factor receptor 2 (FGFR2) also known as CD332 has two naturally occurring isoforms FGFR2IIIb and FGFR2IIIc, created by splicing of the third immunoglobulin-like domain. FGFR2IIIb is predominantly found in ectoderm derived tissues and endothelial organ lining, i.e. skin and internal organs. FGFR2 has important roles in embryonic development and tissue repair, especially bone and blood vessels. Like the other members of the Fibroblast growth factor receptor family, these receptors signal by binding to their ligand and dimerisation (pairing of receptors), which causes the tyrosine kinase domains to initiate a cascade of intracellular signals. As mentioned, FGFR2 mutations are associated with craniosynostosis syndromes, which are skull malformations caused by premature fusion of cranial sutures and other disease features according to the mutation itself.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of FGFR2 and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size: 92 KDa
Observed band size: 100, 150 KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FGFR2 Mouse mAb at 1:500 dilution
Lane 1: MCF7 whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: A431 whole cell lysate
Lane 6: A549 whole cell lysate
Lane 7: NIH/3T3 whole cell lysate
Lane 8: Jurkat whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FGFR2 antibody. Counter stained with hematoxylin., ICC staining of FGFR2 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Fibroblast growth factor receptor 2; FGFR-2; K-sam; KGFR; Keratinocyte growth factor receptor; CD332; FGFR2; BEK; KGFR; KSAM

Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of FGFR2.

Conjugate: Unconjugated

Modification: Unmodified