IL-1 beta Polyclonal Antibody

Catalogue Numbers: BS67482-50, BS67482-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P01584

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses . It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets . Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form . Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of IL-1 beta protein.

Extra Notes: Western blot analysis of IL-1 beta expression in Jurkat (A), Myla2059 (B), Raw264.7 (C) whole cell lysates.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-1 beta. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified