HSP60 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9002-50, MB9002-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10809

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB: 1:2000 IHC/ICC: 1:200

Background: HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker forr γ/δ T cell recognition.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HSP60 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP60 Mouse mAb at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: PC12 whole cell lysate
Lane 5: F9 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin., ICC staining HSP60 inHela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HSPD1; HSP60

Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to HSP60.

Conjugate: Unconjugated

Modification: Unmodified