CD31 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0199P-50, NCP0199P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P16284

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:5000~1:10000
IP 1:50 - 1:200

Background: CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD31 polyclonal antibody detects endogenous levels of CD31 protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD31 polyclonal antibody at 1:5000 dilution
Lane 1: Jurkat whole cell lysate (30ug)
Lane 2: THP-1 whole cell lysate (30ug)
Lane 3: A549 whole cell lysate (30ug), Immunoprecipitation of THP-1 cell membrane lysates using CD31 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD31 pAb #NCP0199P.

Alternative Name: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1

Immunogen: Recombinant protein of human CD31.

Conjugate: Unconjugated

Modification: Unmodified