CD171/N-CAML1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0182P-50, NCP0182P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P32004

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:1000~1:2000
IP 1:50 - 1:200

Background: Neural cell adhesion molecule L1 (NCAM-L1/L1CAM) is a single pass transmembrane glycoprotein member of the immunoglobulin superfamily, containing six amino-terminal extracellular Ig-like domains followed by five fibronectin type-III domains. NCAM-L1 is mainly expressed in the brain, and plays an important role in the developing nervous system, with involvement in neurite fasciculation and outgrowth, myelination, neuronal migration, and neuronal cell adhesion. Mutations in the NCAM-L1 gene cause varying degrees of neurological disease including X-linked hydrocephalus, MASA syndrome, spastic paraplegia type 1, and X-linked corpus callosum agenesis, together known as L1 syndrome. Apart from the nervous system, NCAM-L1 is overexpressed in many cancers and supports a poor prognosis by facilitating aggressive tumor growth, metastasis, and chemoresistance.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD171/N-CAML1 polyclonal antibody detects endogenous levels of CD171/N-CAML1 protein.

Bioworld Molecular Weight: ~ 200 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD171/N-CAML1 polyclonal antibody at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate (30ug)
Lane 2: SGC7901 cell membrane lysate (24ug), Immunoprecipitation of SGC7901 cell lysates using CD171/N-CAML1 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using CD171/N-CAML1 pAb #NCP0182P.

Alternative Name: Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5

Immunogen: Recombinant protein of human CD171/N-CAML1.

Conjugate: Unconjugated

Modification: Unmodified